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Ligation variations and how to transform and create bioluminescent E. coli.
Ligation variations and how to transform and create bioluminescent E. coli. Methods
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Ligation variations and how to transform and create bioluminescent E. coli. Methods
Background & Purpose (approx. 1 paragraph)
• Provides information for readers to understand the scope of the report.
• Background information is supported by citations (and is not simply
copying the lab manual!)
• State the purpose of this research and your predictions (hypothesis).
Focus on the Ligation variations, despite the fact the overall goal was
to transform and create bioluminescent E. coli.
Methods
Ligation variations and how to transform and create bioluminescent E. coli. Methods
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• Include methodological information that is helpful for the reader to
understand the story/interpret the results. • Cite the lab manual as a
place for someone to go for detailed steps.
• You should include information on the strain of competent cells you
used, the plasmid vector including the antibiotic resistance it confers
to the transformed bacteria.
• Overall procedure, what was varied. No volumes or small details. •
Enough of an overview so the reader can interpret results.
Results
Ligation variations and how to transform and create bioluminescent E. coli. Methods
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• Using pooled class data – you are allowed to choose, present results,
including descriptive statistics (e.g. standard deviations) and
statistical analysis. The results you present should be sufficient to
support the research purpose.
• Figures and tables should be clear and easy to interpret. (A table of
class data may be added as the third page of supplementary data)
• Describe the results. Recall the Why? How? What? Where? Components of
a good description.
• Describe relevant differences (e.g. quantify differences).
Conclusions
Ligation variations and how to transform and create bioluminescent E. coli. Methods
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• Explain relevant differences/trends – why do you think these were the
results? Focus on the molecular explanation (molecular with respect to
the ligation reactions).
• Claim – data that supports the claim – reasons why the data supports
it. Reasons should focus on the relevant molecular/biological reasons.
• If there were unexplained/unusual results in the pooled data or your
own data, briefly explain why you think these may have occurred.
• Do these results lead to a generalizable conclusion about cloning
using restriction digest and ligation into pGEM?
• What would you do differently in another experiment to expand on the
results here? Why?
Citations and references
Must be cited in the written portion of the report. Cite using either
numbers [1] or name and year [Cole, 2015].
